Posted in DNA-protein interactions, Gene interactions, epistasis, gene regulation on March 17, 2008 | No Comments »
I learnt about epistasis in twelfth year of schooling. It was first used by Bateson to describe the masking of one allele(variant of a gene) at a locus masking another allele at a different locus. But today I see this word used in different contexts and I was really confused about its meaning, so [...]
Posted in DNA-protein interactions, RNA POlII, gene regulation, transcription, transcription factory, tagged FISH, RNA pol II, transcription, transcription factory on February 19, 2008 | No Comments »
Last week I blogged on the talk by Peter Fraser where he showed evidence for the existence of transcription factories. He showed pictures as follows to demonstrate the theory of transcription factory.
Fig. 1. Transcription factories are concentrated foci of active RNA polymerase. Immuno-detection of the hyper-phosphorylated form of RNAPII reveals their [...]
Posted in DNA-protein interactions, gene regulation, transcription, transcription factory on February 7, 2008 | No Comments »
Today we had a very fascinating story by Peter Fraser (from the Babraham Institute, Cambridge), suggesting that genes migrate to specialized sites called transcription factories in the nucleus for transcription. These transcription factories are nothing but discrete foci in the nucleus containing high concentrations of RNA polII(the eukaryotic polymerase responsible for transcription of protein-coding [...]
Posted in DNA-protein interactions on July 3, 2007 | No Comments »
Today I went to a wonderful seminar by Sebastian J Maerkl. He gave a talk on the a high-throughput microfludic platform that measures the binding affinity of transcription factors.
Transcription factors are protein molecules that bind to the DNA molecule and help to copy the information(genes) in the DNA to RNA and proteins. These transcription factors [...]
